Unexpected quality controls for RNA-seq
It is amazing how many things can go wrong during sequencing. In my experience, samples mix-ups and contaminations are some of the most common. And then of course there are those mysterious ones, for which no one knows what went wrong. Here are few RNA-seq control measures you might not have thought of, but which has helped us in the past to identify sources of faulty results: Antisense transcription. Sure you check the rate of antisense transcription, but did you know that t