Transcription dynamics using RNA-seq
Updated: Feb 25, 2019
They called it "RNA velocity" - the scientists from Karolinska Institute and Harvard Medical School who developed a novel approach to determine whether the transcription of the gene just started or has been going on for a while. What I love about this method is that it doesn't require any additional steps in sample preparation or sequencing - it is utilizing the data that we have often discard as garbage - the intronic reads. If gene transcription just started, majority of transcripts would me still not processed, and therefore the intronic reads will appera more often.